Using MUMmer 3.9.4

Question: Using MUMmer 3.9.4

21 months ago by

nancydong20 • 80 wrote:

Hello everyone!

I am trying to install MUMmer 3.9.4 on Ubuntu, following the instructions given here: https://github.com/gmarcais/mummer/blob/master/INSTALL.md

When I try to run nucmer, I get this error message:

nucmer: error while loading shared libraries: libumdmummer.so.0: cannot open shared object file: No such file or directory

I have tried to look it up online, but can't seem to find anything.

Any help would be greatly appreciated!

Nancy

mummer software error • 1.3k views

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modified 11 months ago by Biostar ♦♦ 20 • written 21 months ago by nancydong20 • 80

Why don't you just install the package? sudo apt-get install mummer

ADD REPLY • link written 21 months ago by kloetzl • 990

Because that version of MUMmer has a query size limit, and my genome files are twice the size of that. I have tried the make CPPFLAGS="-O3 -DSIXTYFOURBITS" get-around, but it doesn't work.

The 3.9.4 version says that it increased the size limit to 120TB, so I wanted to see if this works.

ADD REPLY • link written 21 months ago by nancydong20 • 80

20 months ago by

Bartosz Ulaszewski • 60

Poland

Bartosz Ulaszewski • 60 wrote:

@nancydong20 I had the same problem as you, I did these steps and it helped:

This is critical: Uninstall the existing MUMmer with Synaptic Package Manager. (If you install with sudo apt-get install mummeryou get v 3.22 and it is still called by any software which you use. This is why you cannot use the option CPPFLAGS="-O3 -DSIXTYFOURBITS" to install 64-bit Mummer).
Download the stable MUMer distro 3.23 from https://github.com/gmarcais/mummer
Extract the package
Install MUMer with this code:
cd MUMmer3.23
mkdir aux_bin
make check
make install CPPFLAGS="-O3 -DSIXTYFOURBITS"
mummerpath=pwd
cd ..
export PATH=$mergerpath:$mummerpath:$PATH

In the end you will get:

(maximum reference length is 2305843009213693948)
(maximum query length is 18446744073709551615)
process 6420479 characters per dot

My OS Ubuntu 14, 64 bit. Hope it helps.

ADD COMMENT • link modified 20 months ago • written 20 months ago by Bartosz Ulaszewski • 60

And one more thing in step 9 pwd should be between this symbol `

ADD REPLY • link written 20 months ago by Bartosz Ulaszewski • 60

Thank you SOOO much! It worked!

ADD REPLY • link written 20 months ago by nancydong20 • 80

21 months ago by

nancydong20 • 80

nancydong20 • 80 wrote:

Actually an update, I somehow got over the libumdmummer.so.0 issue, but somehow I see that the size limit still has not changed.

The genomes that I am trying to align on about 1GB each. Should I use MUMmer? I seem to read in other threads that MUMmer can be very memory-intensive if the files are large.

ADD COMMENT • link modified 21 months ago • written 21 months ago by nancydong20 • 80

1GB genomes should fit into (signed) 32-bit integers. Depending on how MUMmer handles the reverse complement, deactivating that, might give you an extra spare bit.

IIRC, Mummer uses ~7 byte per input nucleotide for the suffix tree. That should give you an estimate of how much memory you will need.

ADD REPLY • link written 20 months ago by kloetzl • 990

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