I have 5 spikes (short sequence) and 4 samples (RNA-SEQ), one of them is control and 3 different treatment. I used different concentration for each spike but concentration of each spike in each sample is equal. now I counted the spikes in each sample. in two files the counts are almost the same but the other two samples have different spike counts. meaning in one of them they are 9 times less than control (comparing all 5 spikes between two samples) and the other one is 3 times less than control. now I am going to normalize the read counts using spikes? do you know how I can do that?
Question: read count normalization for spike
3.9 years ago by
Sara • 150
Sara • 150 wrote:
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