Hi all, I am working with Human amplicon data and have been using bwa mem (0.7.15) up to now. Unfortunately, on some datasets the memory consumption exceeds the 8GB RAM I have at spare.
Can someone tell me why this is the case?
I have already tried to modify the parameter (-c or -t) but none of them made it able to run it. Could it be caused by parts of the data aligning to repeat regions/low complexity regions?
If I cannot find a solution to it, I will switch to an alternative aligner that does not need that really high amount of RAM.