Entering edit mode
7.1 years ago
Muhammad Usman
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0
Hi Everyone, can anyone answer the question ? which genes in the Eukaryotes contains no intron & which genes in the prokayrotes contains intron ?
I guess it will be tricky to have a correct overview of such cases. Indeed, in procaryotes most of annotation tools looks at Orf and are not allowing to detect spliced genes. And RNAseqs that could help for such task are still often absent. For eucaryotes many false positives exist, indeed if the annotation tools are not correctly calibrated or use noisy RNAseq you will get lot of fragmented genes,noise (repeats, piece of pseudogenes, etc) seen as single exon genes. You will have to be strict on criteria you decide to use to select them.
Edit: I forgot to say for the eukaryotes that non-intronic CDS doesn't mean non-intronic gene. Depending on the UTRs annotation you may detect single exon genes that are not.
How have you tried to solve this question yourself? I'm also not sure if this question is bioinformatics, but it could be solved using bioinformatics so let's keep it for now.
But this is elementary biology. Prokaryotes don't have splicing.
While generally true that may be a bit of strong statement.
Consider this review and that.
Definitely interesting, wasn't aware of that.
Could add it to the list of things which are oversimplified during biology courses...
this help me in my assignment , Thankyou btw :)
but professor gives us assignmnent to find out these out these which 2/3 contains intron ... and the most important which m not getting from any forum "which gene in Eukaryptes do not contains intron" ... P.S m BS level student
I'm not sure if I understand what you mean by that.
Is this assignment from a bioinformatics course?