Question: Methylation Analysis (Bisulfite sequencing)
gravatar for mks002
2.1 years ago by
Bangalore. India
mks002160 wrote:

Hello all,

Presently I am working on Bisulfite sequencing data for three sample, After primary Analysis using the Bismark tool. I got a bedgraph output. The output bedgraph shows position wise methylation call and the read depth.

For the further Analysis, i wanted to know what should be a better procedure for studying Differential methylation.

Would it be wise choice if we first check the methylation call at different sites ( promoter-TSS, intergenic, exon and TTS). or if would it create a wrong interpretation as the methylation calls are position wise.

Would be thankful for your kind replies.

sequencing next-gen genome • 1.2k views
ADD COMMENTlink modified 21 months ago by hongbo91920 • written 2.1 years ago by mks002160
gravatar for hongbo919
21 months ago by
hongbo91920 wrote:

Hi, we have developed a new software for de novo identification of DMRs from various BS-Seq data including WGBS. Maybe it is useful for you. Best wishes! :)

ADD COMMENTlink modified 21 months ago • written 21 months ago by hongbo91920
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