Question: Methylation Analysis (Bisulfite sequencing)
1
gravatar for mks002
2.6 years ago by
mks002190
Bangalore. India
mks002190 wrote:

Hello all,

Presently I am working on Bisulfite sequencing data for three sample, After primary Analysis using the Bismark tool. I got a bedgraph output. The output bedgraph shows position wise methylation call and the read depth.

For the further Analysis, i wanted to know what should be a better procedure for studying Differential methylation.

Would it be wise choice if we first check the methylation call at different sites ( promoter-TSS, intergenic, exon and TTS). or if would it create a wrong interpretation as the methylation calls are position wise.

Would be thankful for your kind replies.

sequencing next-gen genome • 1.4k views
ADD COMMENTlink modified 2.3 years ago by hongbo91920 • written 2.6 years ago by mks002190
1
gravatar for hongbo919
2.3 years ago by
hongbo91920
hongbo91920 wrote:

Hi, we have developed a new software for de novo identification of DMRs from various BS-Seq data including WGBS. Maybe it is useful for you. http://fame.edbc.org/smart/ Best wishes! :)

ADD COMMENTlink modified 2.3 years ago • written 2.3 years ago by hongbo91920
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