I have a BlastNT.xml file and I tried to perform KEGG and Seed analysis using the kegg and seed mapping files gi2kegg-Aug2016X-ue.bin, gi2seed.map respectively. Since It Is a BlastNT file there is no Ref SEQ Id. I used the GI id.
Setting Min score as 50, Min support 1 and LCA percent 100, I ran the Megan 5 Software. But all I could find after the run was "no hits" and "not assigned" for both Kegg and seed analysis.
Anyone please help me out. I am a newuser of Megan.
Below is the file contents of my BlastNT.xml file <hit_num>1</hit_num> <hit_id>gi|209529865|gb|CP001189.1|</hit_id> <hit_def>Gluconacetobacter diazotrophicus PAl 5, complete genome</hit_def> <hit_accession>CP001189</hit_accession> <hit_len>3887492</hit_len> <hit_hsps> <hsp>
Thanks in advance!