Question: Protein selection from BLASTp result.
0
gravatar for hasanahfaizatul
2.1 years ago by
hasanahfaizatul0 wrote:

hi. i want to ask, if i want to select protein from a blastp result , which parameter should i look closely before i select a protein. evalue, percent identity or score? and if there is E-Value = 0, is it okay to choose that e-value or i have to take a look at the e-value that is near 0?

thank you.

blast alignment sequence • 481 views
ADD COMMENTlink modified 2.1 years ago by jrj.healey12k • written 2.1 years ago by hasanahfaizatul0
1
gravatar for jrj.healey
2.1 years ago by
jrj.healey12k
United Kingdom
jrj.healey12k wrote:

I'm not sure what the question is exactly, but it sounds like you're asking what the best result is?

Your best match from BLAST will have an E-Value as close to 0 as possible (0 is as good as it gets), and ideally will have a large Bitscore. You may need to look at the query coverage etc however if you're just interested in a particular sub-region of the protein etc.

ADD COMMENTlink written 2.1 years ago by jrj.healey12k

okay thank you so much.

ADD REPLYlink written 2.1 years ago by hasanahfaizatul0
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