Question: DNA methylation 450k data - minfi - preprocessFunnorm - CNV cases vs non-CNV controls
0
gravatar for Thomas
15 months ago by
Thomas0
Thomas0 wrote:

Hello,

I am looking for DNA methylation differences in a case/control experiment; all samples were run on 450k arrays. I am using the minfi package to analyze the data, and normalizing it with the preprocessFunnorm function. All the cases have a copy number variation (CNV) encompassing multiple genes.

1) Should I expect to see difference in methylation for the probes contained within the CNV (due to the different copy number) or would the difference in probe intensity between cases and controls be normalised?

2) If the observed difference in methylation observed is due to the copy number difference, is there an approach to look at the methylation of these CNV probes per chromosomal copy?

Thanks in advance,

Thomas

ADD COMMENTlink modified 15 months ago • written 15 months ago by Thomas0
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 586 users visited in the last hour