Question: How does coverage of all contigs varies?
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20 months ago by
BioGeek130
BioGeek130 wrote:

How does coverage of all contigs varies? Why contaminants contigs have various coverage in an assembly?

depth coverage ngs reads • 591 views
ADD COMMENTlink modified 20 months ago by h.mon21k • written 20 months ago by BioGeek130
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This question could use more information and context, please elaborate.

ADD REPLYlink written 20 months ago by WouterDeCoster35k
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gravatar for h.mon
20 months ago by
h.mon21k
Brazil
h.mon21k wrote:

Read about coverage here, here, here and here. There are plenty of other sources, just google for "sequencing coverage" and variants.

Contaminants usually have lower and more variable coverage because they are non-obvious, small introductions of extraneous material into the source material, which can occur at many steps from biological sample collection to library preparation to sequencing.

ADD COMMENTlink modified 20 months ago • written 20 months ago by h.mon21k
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