Indels by Sanger
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4.6 years ago

Can I detect small indels from Sanger sequencing results? How to perform the analysis?

indels sanger capillary • 2.4k views
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4.6 years ago

Since small indels will cause shifts in your chromatogram these can be detected. It might be a bit problematic to identify which event exactly causes the shift. Nice software is for example novosnp, developed a while ago in the department I'm working.

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h.mon 33k

There is also SeqDoC.

Staden - more specifically, the Gap4 module, which has a contig editor - may be used for variant calling, although it is not designed for this, and it a bit difficult to learn.

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4.6 years ago
trausch ★ 1.6k

Indigo can decompose heterozygous InDels in Sanger traces.

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