Question: Indels by Sanger
0
gravatar for generoso.ianniciello
2.2 years ago by
generoso.ianniciello0 wrote:

Can I detect small indels from Sanger sequencing results? How to perform the analysis?

sanger indels capillary • 1.5k views
ADD COMMENTlink modified 2.2 years ago by trausch1.3k • written 2.2 years ago by generoso.ianniciello0
1
gravatar for WouterDeCoster
2.2 years ago by
Belgium
WouterDeCoster39k wrote:

Since small indels will cause shifts in your chromatogram these can be detected. It might be a bit problematic to identify which event exactly causes the shift. Nice software is for example novosnp, developed a while ago in the department I'm working.

ADD COMMENTlink written 2.2 years ago by WouterDeCoster39k
0
gravatar for h.mon
2.2 years ago by
h.mon26k
Brazil
h.mon26k wrote:

There is also SeqDoC.

Staden - more specifically, the Gap4 module, which has a contig editor - may be used for variant calling, although it is not designed for this, and it a bit difficult to learn.

ADD COMMENTlink written 2.2 years ago by h.mon26k
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gravatar for trausch
2.2 years ago by
trausch1.3k
Germany
trausch1.3k wrote:

Indigo can decompose heterozygous InDels in Sanger traces.

ADD COMMENTlink written 2.2 years ago by trausch1.3k
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