I saw this plot
Normalization factors for the fruit set RNA-Seq data depending on corresponding library sizes. All three studied normalization methods are carried out with default settings. For all three methods, regression (dashed) lines are estimated from a simple linear regression modeling the relationship between default normalization factors and library sizes. Color key: TMM, RLE, and MRN are respectively colored in green, blue, and red. Key to symbols: Bud, Ant, and Pos stages are respectively drawn with circles, squares, and triangles.
Question: What is the relationship between
library size and
normalization factor? What does it mean if the
regression line have