**4.0k**wrote:

I saw this plot

doi.org/10.3389/fgene.2016.00164

Normalization factors for the fruit set RNA-Seq data depending on corresponding library sizes. All three studied normalization methods are carried out with default settings. For all three methods, regression (dashed) lines are estimated from a simple linear regression modeling the relationship between default normalization factors and library sizes. Color key: TMM, RLE, and MRN are respectively colored in green, blue, and red. Key to symbols: Bud, Ant, and Pos stages are respectively drawn with circles, squares, and triangles.

**Question:** What is the relationship between `library size`

and `normalization factor`

? What does it mean if the `regression line`

have `R^2`

of `0.9`

?

**0**• written 3.3 years ago by Deepak Tanwar •

**4.0k**