Hi, A lab member has the results cvs file from DESEQ2. I am trying to recreate the results given that she lost her initial runs as her hard drive crashed. However, I get many less significant genes (100s). I think I am using the same counts and procedure as she used. The results have the same base mean for the overlapping sets. However the log2fold difference are different. I feel the dispersion calculations must be distinct between what I am running and what she has run. Has this changed between versions? Maybe I am using different parameters then her? I have not tried different modification parameters yet to try to see if I can get back her results? Can someone suggest parameters/techniques or what to look at to diagnose this problem?
Question: number of significant genes different between 2 different runs of DESEQ2
2.1 years ago by
modockesner • 0
modockesner • 0 wrote:
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