Tutorial:Galaxy Tutorial on ChIP-seq
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7.1 years ago
anton ▴ 30

A new Galaxy ChIP-seq tutorial is here - https://galaxyproject.org/tutorials/chip/ More tutorials are coming...

galaxy ChIP-Seq • 3.9k views
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A ChIP-exo tutorial is coming...

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7.1 years ago
anton ▴ 30

In this tutorial we analyze a two replicate ChIP-seq experiment. The tutorial outline is:

  • pre-process sequencing reads
  • map reads
  • post-process mapped data
  • assess quality and strength of ChIP-signal
  • display coverage plots in a genome browser
  • call ChIP peaks with MACS2
  • inspect obtained calls
  • look for sequence motifs using MEME
  • look at distribution of enriched regions across genes.
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