Question: remove effect of factors in differential analysis
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gravatar for hellocita
2.2 years ago by
hellocita10
hellocita10 wrote:

Assume there's two factors that affect the metabolite compounds content of my data: Factor A and Factor B, Factor Ahas three level and Factor B has two level, so there is 3*2 groups, and all in one experiment batch. I only want to get the compounds related to the Factor A, however Factor B seems to have dominate effect on the data(from unsupervised dendrogram and PCA).

I have tried two approaches to analysis differential compounds: First way is use ANOVA to analysis full model(content ~ Factor A + Factor B) and reduced model(content ~ Factor A and Factor B). Second way is use multiple t.test to analysis different group of Factor B, and after p-adjust, use the overlap of their differential sets. The third approach is to use permutation F test set p < 0.05 and use F cutoff to estimate FDR and make FDR< 0.05 to get the differential compound set.

I tried both ways and get the differential compound sets(p<0.01, FDR<0.05), however, on the dendrogram the same species samples still cluster together using first or third approach, and the second approach which use multiple t.test, since the sample size is two small there is significant result.

Is there any other better ways to do this?

And thanks in advance for your advice :)

R • 628 views
ADD COMMENTlink modified 2.2 years ago • written 2.2 years ago by hellocita10
1

I think you should give a little bit more context.

Like how did you calculate the differential (expression?) analysis. Which model did you use ...

ADD REPLYlink written 2.2 years ago by VHahaut1.1k

thanks! I have re-edit it and hope it's more clear now!

ADD REPLYlink written 2.2 years ago by hellocita10

full model(content ~ Factor A - Factor B -interaction(FactorA, Factor B) )

ADD REPLYlink modified 2.2 years ago • written 2.2 years ago by theobroma221.1k
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gravatar for theobroma22
2.2 years ago by
theobroma221.1k
theobroma221.1k wrote:

I have to edit / update this tutorial but it's very useful for what you are doing. Check it out: It's about the journey, and not the destination

ADD COMMENTlink written 2.2 years ago by theobroma221.1k
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