Hi, We have sequenced 250 bp read length library at 100 X 2 cycles on Miseq by mistake, because of which the R1 and R2 fastq files are of 100 bp in length each and are non-overlapping. How to align these fastq files to reference genome. Can we align R1 as single end sequencing file to generate .BAM for R1. Also reverse complement R2 and do the same. Later we can merge two .BAM files for downstream processing. Any suggestions?? Thanks in advance.
Question: How to align non overlapping fastq files
3.2 years ago by
Rongen • 10
Rongen • 10 wrote:
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