Question: Aligning metagenomic reads (OTU representatives) to my phylogenetic tree ~AND~ evaluating how multiple samples align
gravatar for seccon
3.5 years ago by
seccon0 wrote:


I am working with amino acid sequences.

I got a fasta file containing reference sequences and a txt file giving the categorization (domain, subdomain, nickname etc etc etc.) for each sequence. First thing, I need to construct a tree with these sequences to check that they group according to the categories they belong to. Example: sequences belonging to domain A group together, sequences belonging to subdomain A1 group together and so on.

Then... I got a fasta file containing OTU representatives derived from metagenomic reads AND a OTU count table declaring how many of each of these representatives are present in each one of my 20 samples. I need to align these OTU sequences to the previously constructed tree ~AND~ consider the per-sample count, to check the categories each sample is enriched for.

What softwares should I use?

Thank you!

ADD COMMENTlink modified 10 months ago by Biostar ♦♦ 20 • written 3.5 years ago by seccon0
Please log in to add an answer.


Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 869 users visited in the last hour