Question: prediction form DNA (A,B,Z) by sequence
gravatar for miravet65
24 months ago by
miravet650 wrote:

hi i'm looking for a tool or online tools that creates predictions of secondary structure .i have three different sequence DNA and i checking form of these . is there site or tools for predict DNA formation example this sequence produce Z DNA or A,B i want checking interaction protein and DNA but before start my work ,i need to determine form and structure DNA

sequencing • 1.0k views
ADD COMMENTlink modified 24 months ago by jotan1.2k • written 24 months ago by miravet650
gravatar for Lars Juhl Jensen
24 months ago by
Copenhagen, Denmark
Lars Juhl Jensen11k wrote:

It is crucial to realize that the form of DNA is dynamic. You should not think of it as something static; I am not aware of there being any regions in the genome that are permanently in A- or Z-form.

To my understanding, conversion of DNA from the default B-form to A-form primarily happens as a response to dehydration, presumably to protect the DNA. I am thus not sure that A-form is really something you need to worry about; if you are looking for protein-DNA interactions in happily growing cells, I think you can safely ignore A-DNA.

Because Z-DNA forms a left-hand helix (as opposed to the right-hand A- and B-DNA), the formation of Z-DNA happens in response to negative supercoiling. Some work was done long ago on predicting the propensity of different DNA sequences to form Z-DNA when negatively supercoiled (Ho et al., 1986). I am not aware of a tool that you can download, though.

However, I think it is fairly safe to assume that most DNA is in B-form most of the time. If the protein you are interested binds to B-form DNA, I think you are on bit of a wild goose chase here.

ADD COMMENTlink written 24 months ago by Lars Juhl Jensen11k

thanks. when protein binding to DNA or even protein reaction occur in hydrophobic condition and dehydration so DNA form is Aform but my DNA sequence is CG-rich that is a main factor for Z form, i think your idea is correct in cells but occur reaction in servers for docking or MD by different form of DNA but im not sure!!!!

ADD REPLYlink modified 24 months ago • written 24 months ago by miravet650
gravatar for jotan
24 months ago by
jotan1.2k wrote:

This database predicts non-B DNA structures

Non-B DB v2.0: a database of predicted non-B DNA-forming motifs and its associated tools.

But be careful. Lars Juhl Jensen is absolutely right, you need to be very careful in interpreting a prediction

ADD COMMENTlink written 24 months ago by jotan1.2k

thanks . I am very grateful to have introduced a very good site. My problem was partly solved

ADD REPLYlink written 24 months ago by miravet650
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