How to find number of genes in Agilent Protocol
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8.6 years ago
haiying.kong ▴ 360

I would like to find out how many genes are in the targeted area for a given Agilent protocol for WES. Agilent website gives only the number of probes, and among the dataset I downloaded, there is one txt file, like: S07604624_Targets.txt gives a list of names. But the list of names is mixture of gene symbol, Ensembl Transcript ID, GenBank ID, Refseq ID, and about 22% of them are not having corresponding gene symbols if I use the database: org.Hs.eg.db.

  [1] "KJ891129"        "X98054"          "ENST00000369829" "NM_173803"
  [5] "BC172493"        "ENST00000371910" "ENST00000491059" "NM_001301039"
  [9] "CCDS60146.1"     "ENST00000559981" "AK098124"        "CU688405"
 [13] "ENST00000511269" "ENST00000522231" "ENST00000449052" "KJ895283"
 [17] "AK075206"        "CCDS4839.1"      "ENST00000396353" "AB463204"
 [21] "AF338924"        "AK124803"        "KJ902737"        "CCDS46147.1"
 [25] "L38251"          "AK097145"        "CCDS47799.1"     "NM_032125"
 [29] "ENST00000409413" "AK294470"        "AB749845"        "U93181"
 [33] "ENST00000502829" "CU689180"        "ENST00000329451" "AK024678"
 [37] "CCDS7428.1"      "ENST00000515224" "AK296850"        "ENST00000458599"
 [41] "AB464008"        "Z15108"          "AK314242"        "BC022387"
 [45] "ENST00000531291" "ENST00000374450" "BX647401"        "ENST00000477633"
 [49] "KJ903845"        "ENST00000529099" "NM_014423"       "CCDS2110.1"
 [53] "CCDS13496.1"     "NR_026580"       "CCDS46257.1"     "NM_207355"

I thought this is a basic information Agilent would provide. Where can I find this information, or how can I calculate this?

WES • 1.4k views
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Maybe you can ask an Agilent sales person? They always seem to know everything.

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I don't know about that... They keep on calling me if I want to use their kit. Apparently, they don't know yet how to f*ck off.

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