Question: Interpreting Chip Seq Results
2
gravatar for benjyrolls
3.2 years ago by
benjyrolls70
benjyrolls70 wrote:

Hello

I performed a ChIP Seq experiment and performed these steps MACS, CEAS, Gene Ontology Enrichment with GREAT .

I have some enriched terms as well as enriched pathways such as PTEN Signalling pathway, WNT pathway etc.

From my information how can I find the genes which are up or down regulated and the effects they are having on these pathways?

How far can I go with my ChIP-Seq analysis after Gene Ontology Analysis to find the meaning of my enriched terms and pathways?

Thanks Benjy

great ceas chip-seq macs2 • 1.3k views
ADD COMMENTlink modified 3.2 years ago by Devon Ryan95k • written 3.2 years ago by benjyrolls70
1

It is really difficult/ not possible to give suggestions based on the information you provided. Would be helpful if you give more details like which histone mark / TF ChIP data you are analysing, which disease context the analysis based on, because PTEN, WNT pathway may not be useful for some diseases. Do you have RNA-seq data or microarray gene expression data?

btw, what is you aim/hypothesis behind analysis?

ADD REPLYlink written 3.2 years ago by venu6.6k
4
gravatar for Devon Ryan
3.2 years ago by
Devon Ryan95k
Freiburg, Germany
Devon Ryan95k wrote:

You can find the up/down regulated genes by performing RNAseq. You're done analysing the ChIPseq data, do the wetlab experiment.

ADD COMMENTlink written 3.2 years ago by Devon Ryan95k
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