I hope you all are doing good. My question is that I have log2 gene expression data of four different time span (30min, 1hr, 2hr and 4hr). I want to calculate the Fold change (FC) and P-value for it, Can I calculate FC individually for each time. For example (30min_FC,1hr_FC, 2hr_FC and 4hr_FC)? The number of columns is (10X4=40), in that case, we have 40 different fold change w.r.t time and similarly P-value. What can I do in this case?