Question: possion distribution of ChIP-seq
0
gravatar for Ben
2.6 years ago by
Ben50
Ben50 wrote:

I want to use MACS to call peaks, the MACS used possion distribution to detect significant peaks. Does anyone know how to used possion distribution to detect peaks?

chip-seq • 1.0k views
ADD COMMENTlink modified 2.6 years ago • written 2.6 years ago by Ben50
1

It is spelled "Poisson", with the capital P :)

ADD REPLYlink written 2.6 years ago by RamRS25k

You never know: https://www.ncbi.nlm.nih.gov/pubmed/?term=possion
"Multivariate possion regression analysis was applied ..." :)

ADD REPLYlink modified 2.6 years ago • written 2.6 years ago by PoGibas4.8k
0
gravatar for Devon Ryan
2.6 years ago by
Devon Ryan93k
Freiburg, Germany
Devon Ryan93k wrote:

It's generally presumed that a background (either an input sample or non-enriched regions) will have signal following a Poisson distribution. Consequently, one can track the lambda from that (see the --llocal and --slocal options) see if a given spot has siginificantly more signal than expected given that distribution. This is then given a p-value accordingly and you have your peak.

Edit: The wiki from MACS2 may be helpful.

ADD COMMENTlink modified 2.6 years ago • written 2.6 years ago by Devon Ryan93k
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