Question: how to design primers for AC112229.1 pseudogene and no for his parental gene ?
gravatar for reimco2
3.3 years ago by
reimco20 wrote:

Hi every one, I'm trying to design primers for this pseudogene. But these peseudogene are not available in nbci nucleotide it's take a part of a clone, and his parental gene is (ZNF532). when i toke the sequence of pesudogene and design primers. primers cover or amplified the parental gene and not pseudogene. How to design primers in this case. Hope to hear advices from you.. Thanks a lot..

gene • 1.0k views
ADD COMMENTlink modified 3.3 years ago • written 3.3 years ago by reimco20

Primers for what? Real-time qPCR? Regular genomic DNA amplification?

ADD REPLYlink written 3.3 years ago by h.mon30k

So sorry. it was a mistake.. thanks .. i need to desing primers for Real-time qPCR( RT-qPCR) and my target is quantify pseudogene expression...thank you for your point.

ADD REPLYlink written 3.3 years ago by reimco20

Please use ADD COMMENT/ADD REPLY when responding to existing posts to keep threads logically organized.

ADD REPLYlink written 3.3 years ago by genomax87k
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