Say I have some probe level and RMA normalized array data. The data is supposedly log2 normalized already. How do I calculate the relative difference in intensity between two probes?
If I understand correctly, it should just be the 2^residual.
probe1 = 1 probe2 = 2 2^(2-1) = probe2 has 2x as much signal as probe1
However for values less than 1, I'm not sure this makes sense:
probe1 = 0.25 probe2 = 0.5 2^(0.5-0.25) = probe2 has 1.19x as much signal as probe2?
Shouldn't it be 2x as well in this instance?