How to annotate the Bacterial genome VCF ?

Question

[k.kathirvel93]

Hi All, I have done De Novo assembly with 5 p.aeruginosa genomes, and i got some Variants in some target genes. Now i want to confirm those variants with using BWA and SamTools. BWA and SamTools were done with those 5 genomes, now i want to annotate my VCF file. How can i Annotate ? Suggestions ?

Thanks in Advance

Answer 1

[Titus]

Hi , you can have a look on VEP there is files to annotate p.aeruginosa : http://bacteria.ensembl.org/info/website/ftp/index.html

Best