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6.1 years ago
seta ★ 1.8k
I used cufflinks package for genome-guided transcriptome assembly, followed by using gffread for converting the produced gtf file to fasta file, but some warnings (like below) appeared during this conversion,
Warning: couldn't find fasta record for 'HSCHR6_MHC_SSTO'! Warning: getSpliced(NULL,.. ) called!
Could you please let me know what's the warning and if it's important?
Seems like genome fasta and genome annotation are from different sources or versions.
But it would be helpful if you provide more details to your question.