What I am trying to do is blast about 50 RNA sequences against the genomes of various organisms (All large genomes for vertebrates, other animals). I am trying to do this locally because it takes an enormous amount of time otherwise.
How I am trying to do this is by running update_blast.pl (a script provided in blast+ to locally download databases via ftp).
The problem is this is taking very long and the files that I am downloading are taking up a lot of space. I'm wondering if there is a more sensible way to do this because the databases are taking up about 100 gb on my computer right now.
Heres how I am running it (If I don't adjust the timeout, it usually doesn't finish)
update_blastdb.pl --timeout 800 nt.
I am also downloading
And have completed
I will want to perform these blast searches for multiple organisms, but each individual blast search will be against one organism
On a side note, I've noticed if I need to ^C certain downloads and restart them, they will finish but not otherwise