Create gene expression matrix from miRNA
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6.9 years ago
landscape95 ▴ 190

I need to create a matrix of miRNA that consists of rows for gene and columns for samples in R. miRNA data is downloaded by TCGAbiolinks.

Your help is really appreciated!

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miRNA • 2.3k views
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Could you be more specific about what you want to do with those and in which language of programmation ?

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I use R programming language. I want to create a correlation network and for further usage. Here is the data of miRNA I downloaded. I update the image on the topic.

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It's unclear which data you currently have. Reads? Alignments? Count tables?

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Here is the data of miRNA I downloaded. [I updated the image on the topic.]

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Or you could be just more informative instead of expecting us to click on anonymous links.

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Yes, I am sorry. To be more specific. I want to express via the image. I updated the image on the topic. Hope you can see it.

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6.9 years ago
h.mon 35k

Did you read this data into R? This is a pretty basic operation. Did you have a look at the data? Do you know which columns you want / need to use for your analysis? See the R helps for:

?read.delim

?read.table

And search for "subset data frame R" on your favorite search engine.

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6.9 years ago
VHahaut ★ 1.2k

It doesn't really tell us for what is it but here is a code in R to get the count data into a matrix:

  • Assuming that this is in a text file (and not excel) that is located on your desktop at this address: "~/Desktop/count.txt"

    a <- read.table("~/Desktop/count.txt", header=T, row.names=1, sep="\t")

    a <- as.matrix(a[,grep("read_count", colnames(a))])

This should probably work.

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