Question

Best splicing variant

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6.9 years ago

valopes ▴ 30

Dear friends,

I need to get the best splicing variant of all soybean transcripts. How can I do that? Can I say that the longest is the "best splicing variant"?

Thanks

rna-seq • 1.2k views

ADD COMMENT • link updated 6.9 years ago by jnoble333 ▴ 20 • written 6.9 years ago by valopes ▴ 30

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Can I say that the longest is the "best splicing variant"?

No, why would that be the best one?
Another method would be to select the most abundantly expressed...

ADD REPLY • link 6.9 years ago by WouterDeCoster 47k

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Sure. This makes more sense. Thank you.

ADD REPLY • link 6.9 years ago by valopes ▴ 30

score 0 · Answer 1 · 2017-05-19

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6.9 years ago

jnoble333 ▴ 20

What do you mean by best? Would it be the most highly expressed variant? It really comes down to what question you are trying to answer with your data set.

ADD COMMENT • link 6.9 years ago by jnoble333 ▴ 20

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Yep, I don't even think the question makes any sense. "best splicing variant"? That has no meaning.

ADD REPLY • link 6.9 years ago by Brian Bushnell 20k