Hi. I am now working on a transposon mutagenesis system and everything seems to work. Most of the plasmids have been lost after a successful conjugation and the insertion gene is detectable by PCR from cell culture. However, considering there are still a tiny copy number of plasmids in some of the cells, I'd like to extract the chromosomal DNA (free from the plasmids) from the cells and test the insertion gene directly from their gDNA. Any kits recommended? Btw the cells are G- Cellulaphaga baltica.
Question: (Closed) How to extract chromosal DNA from G- bacteria?
2.8 years ago by
liu.5687 • 10
liu.5687 • 10 wrote:
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