Question: (Closed) How to extract chromosal DNA from G- bacteria?
gravatar for liu.5687
2.8 years ago by
liu.568710 wrote:

Hi. I am now working on a transposon mutagenesis system and everything seems to work. Most of the plasmids have been lost after a successful conjugation and the insertion gene is detectable by PCR from cell culture. However, considering there are still a tiny copy number of plasmids in some of the cells, I'd like to extract the chromosomal DNA (free from the plasmids) from the cells and test the insertion gene directly from their gDNA. Any kits recommended? Btw the cells are G- Cellulaphaga baltica.

chromosal dna extraction • 515 views
ADD COMMENTlink written 2.8 years ago by liu.568710

Hello liu.5687!

We believe that this post does not fit the main topic of this site.

There is no bioinformatics component to this question. You may want to post over in Biology stackexchange.

For this reason we have closed your question. This allows us to keep the site focused on the topics that the community can help with.

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ADD REPLYlink written 2.8 years ago by genomax78k
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