Question: what library preparation kit was used in sequencing process?
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gravatar for kk.mahsa
3.7 years ago by
kk.mahsa120
kk.mahsa120 wrote:

is there any way to figure out library preparation kit from fastq file (sequenced by illumina)? reads identifiers in fastq file can help me to recognize it?

sequencing next-gen illumina • 795 views
ADD COMMENTlink written 3.7 years ago by kk.mahsa120

You can try to see by FASTQC tool what is the length of one read, if it's PE or SE, maybe there are some adaptors left - and try to fit with Illumina kits.

ADD REPLYlink written 3.7 years ago by agata88800

i did it but there is no adapter.

ADD REPLYlink written 3.7 years ago by kk.mahsa120

how your reads are named? Is it SE or PE? What is the length of read?

ADD REPLYlink modified 3.7 years ago • written 3.7 years ago by agata88800

it is PE reads and 2*100

ADD REPLYlink written 3.7 years ago by kk.mahsa120

When you run fastQC it will also tell you which illumina technology was used (like illumina 1.9 or illumina 1.5+). This is not a clear answer but can allow you to search which are the library preparation kits for such technologies, perhaps there is only one (i'm guessing).

ADD REPLYlink written 3.7 years ago by Macspider3.3k
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