Question: Detecting Variants from Paralogs gene
0
gravatar for danangcrysnanto
3.5 years ago by
danangcrysnanto0 wrote:

Hi All!

I am working on the duplicated genes in Drosophila pseudoobscura. I can found the location of duplicated genes using Flybase and Orthodb. Next, I want to make population genetics analysis of the paralogs, in which that I need to collect the variants in paralogs and checked whether it is evolve faster after duplication.

However, many of the paralogs are a young paralog which mean that there are not so many nucleotide difference between them.

Since my data is genomic reads, Is it possible to map the genetic variation precisely for each paralogs? I tried to map with bowtie2 with --verysensitive option and then following use Haplotype Caller to call the variants. However, I find many NNNN (undertermined base) following variants calling which occur in the duplicated regions. Is there any better way for mapping variants in duplicated regions in the genome?

Any help will be much appreciated

paralogs variants • 996 views
ADD COMMENTlink written 3.5 years ago by danangcrysnanto0

Hi,

You should use BLAST for this kind of work because it is more accurate.

Best

ADD REPLYlink written 3.5 years ago by Titus910
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1843 users visited in the last hour