Question: Tool for BAM coverage without the soft clipping base
0
gravatar for nayshool
18 months ago by
nayshool20
Israel
nayshool20 wrote:

Hi everyone,

I am looking for a software/script which calculate the mean coverage of an area without the soft clipping bases. It's exclude the soft/hard clipping bases and leave only the true annotated bases in the calculation.

thank you

coverage bam softclipping • 636 views
ADD COMMENTlink modified 18 months ago by Pierre Lindenbaum115k • written 18 months ago by nayshool20
0
gravatar for Devon Ryan
18 months ago by
Devon Ryan86k
Freiburg, Germany
Devon Ryan86k wrote:

Almost anything that produces coverage metrics will do what you're describing. As an example, you can use bamCoverage or multiBamSummary from deepTools to do this, though other common choices would be bedtools or even featureCounts.

ADD COMMENTlink written 18 months ago by Devon Ryan86k

I don't want to count the soft clipping reads. Are those software will not count them?

ADD REPLYlink written 18 months ago by nayshool20

Correct, none of those (or even samtools view -c) will count soft-clipped portions of reads.

ADD REPLYlink written 18 months ago by Devon Ryan86k
0
gravatar for Pierre Lindenbaum
18 months ago by
France/Nantes/Institut du Thorax - INSERM UMR1087
Pierre Lindenbaum115k wrote:

GATK depthofcoverage: https://software.broadinstitute.org/gatk/gatkdocs/3.7-0/org_broadinstitute_gatk_tools_walkers_coverage_DepthOfCoverage.php

In most (all?) tools the clipping section is ignored.

ADD COMMENTlink written 18 months ago by Pierre Lindenbaum115k
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