I have to analyze the result of an RNAseq with R. The experiment consisted in growing bacteria in five different culture conditions and then the total RNA of each condition was sequenced. The result they gave me was a table, which looks like this:
Gene ID Reads Condition 1 Reads condition 2 ....(RC3,RC4).. Reads condition 5 GEN_R5 21 56 ......... 44 GEN_R10 182 408 ......... 304 GEN_R15 3 1 ......... 7 . . (3400 genes)
I have found many tutorials to process and analyze this data, but they start from sequence or alignment and I don't understand them very well.. Please, if someone can guide me from the information I have in this table or if you have any tutorial that I can follow, I would greatly appreciate it.