Question: Using RNA seq for genome annotation
0
gravatar for Picasa
2.6 years ago by
Picasa480
Picasa480 wrote:

Hi,

I would like to provide rna seq evidence to Maker for genome annotation.

For the transcriptome building, I have done a reference based assembly with STAR+cufflinks but I am not sure if it is the correct way,

Do you have some experience with this ?

maker annotation • 1.2k views
ADD COMMENTlink modified 2.6 years ago by Ben50 • written 2.6 years ago by Picasa480

Do you want to annotate the genome?

ADD REPLYlink written 2.6 years ago by Ben50

yes, and i want to provide rna seq evidence

ADD REPLYlink written 2.6 years ago by Picasa480

RNA-seq evidenece? I do not understand what you want to express

ADD REPLYlink written 2.6 years ago by Ben50

Well Maker needs (not mandatory but recommended) transcriptome to infer gene with gene predictor.

ADD REPLYlink written 2.6 years ago by Picasa480
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