Trying ballgown stattest with covariates = group for my dataset throws me error. Can please solve based on the details below.
Error in stattest(bg, feature = "transcript", meas = "FPKM", getFC = TRUE, covariate="group") : There must be at least two replicates per group. Make sure covariate is categorical; if continuous, consider the timecourse option, or specify your own models with mod and mod0.
Following is the dataset:
ids,group var1,control var2,sample1 var3,sample2 var4,sample3 var5,sample4 var6,sample5 var7,sample6 var8,sample7 var9,sample8 var10,sample9 var10,sample10 var11,sample11
Can anyone tell me how to design the model for this. ?