Question: Difference between read and contig based taxonomic classification
1
gravatar for BAGeno
4 months ago by
BAGeno20
BAGeno20 wrote:

Hi,

I want to understand the difference between read based and contig based classification. What is the methodology behind these two? Which is more accurate?

ADD COMMENTlink modified 4 months ago by Sej Modha2.0k • written 4 months ago by BAGeno20
1
gravatar for Sej Modha
4 months ago by
Sej Modha2.0k
Glasgow, UK
Sej Modha2.0k wrote:

Read based taxonomic classification is where each read generated from a sequencing method is search against a database of known sequences to find similarity. Contig are longer sequences generated by stitching overlapping reads. Contig based classification is where these longer sequences are search against known sequences to identify similarity and label them taxonomically. For both cases a number of different tools can be used to search against a set of databases. Some examples of such tools are: RiboPicker, BLAST, DIAMOND

ADD COMMENTlink written 4 months ago by Sej Modha2.0k
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