Question: Difference between read and contig based taxonomic classification
gravatar for BAGeno
11 weeks ago by
BAGeno20 wrote:


I want to understand the difference between read based and contig based classification. What is the methodology behind these two? Which is more accurate?

ADD COMMENTlink modified 9 weeks ago by Sej Modha1.7k • written 11 weeks ago by BAGeno20
gravatar for Sej Modha
9 weeks ago by
Sej Modha1.7k
Glasgow, UK
Sej Modha1.7k wrote:

Read based taxonomic classification is where each read generated from a sequencing method is search against a database of known sequences to find similarity. Contig are longer sequences generated by stitching overlapping reads. Contig based classification is where these longer sequences are search against known sequences to identify similarity and label them taxonomically. For both cases a number of different tools can be used to search against a set of databases. Some examples of such tools are: RiboPicker, BLAST, DIAMOND

ADD COMMENTlink written 9 weeks ago by Sej Modha1.7k
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