Question: The zero value in RNA seq
0
gravatar for saj98
22 months ago by
saj98100
saj98100 wrote:

Hello everyone

I have RNA seq FPKM, some of the genes came with zero value for treatment group, but with defined value for the control group. The cuff links read them as significant genes. Can we recognize these genes not express in treatment group but they do so in control group?

Thanks for help

rna-seq • 732 views
ADD COMMENTlink written 22 months ago by saj98100

Hi saj98 - I think it the grammar of your question is not clear - what do you mean by "Can we recognize these genes"?

ADD REPLYlink written 22 months ago by YaGalbi1.4k

Hello Sorry for misunderstanding, my question: can I count these genes even if their value zero in treatment group?

Thanks

ADD REPLYlink written 22 months ago by saj98100

IF fpkm>0 for gene X in control group

AND fpkm=0 for gene X in treatment group

AND p<0.05 (according to cufflinks)

THEN there is a significant difference between treated and control for gene X at the 0.05 significance level

The simple conclusion (with no other information) is that treatment has stopped the expression of gene X

ADD REPLYlink modified 22 months ago • written 22 months ago by YaGalbi1.4k

Thank you so much, you answer was useful for me.

ADD REPLYlink written 22 months ago by saj98100
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