Question: How can I Annotate my miRNA data against MirBase?
1
gravatar for k.kathirvel93
2.1 years ago by
k.kathirvel93190
India
k.kathirvel93190 wrote:

Hi All, Can anyone help? How can I annotate my microRNA data against MirBase. Initially, my microRNA data were mapped against the reference genome by Bowtie2, and sam file were converted into bam and sorted by picard. Now i want to get to know, how do i annotate my bam file against mirbase database. Thanks in advance.

K.Kathir

ADD COMMENTlink modified 23 months ago by Biostar ♦♦ 20 • written 2.1 years ago by k.kathirvel93190

what is "how do i annotated my bam file against mirbase database" ? what kind of output do you need ?

ADD REPLYlink written 2.1 years ago by Pierre Lindenbaum121k

From bam file(s), assuming that there are controls and cases, get a list of deferentially expressed and statistically significant miRNAs. Then you can use miRbase. To get significant miRNA between groups (controls and cases), follow some thing like here: http://miarmaseq.cbbio.es/

ADD REPLYlink written 2.1 years ago by cpad011211k
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