Now, I research that “Comprehensive Identification of Cell Fate Decision-related ncRNAs in Human”. For identification of ncRNA (particularly lncRNA and miRNA), we need firstly that systematically select core reprogramming-related transcript factors (TFs). Then, identifying ncRNAs that associated with these TFs. To calculate differential expression genes, I need two classes of sample: the first is case, and the second is control. I want to class samples by cell type's .obo format files. However, I don’t know how to operate cell type tree file (.obo format file, ff-phase1-prerelease-20130717.obo). The people around me also did not have the experience of dealing with thus data.I think it's very helpful for my experiments with your help. Because it’s the beginning of our research. Do you have some recommended software packages or papers for dealing with .obo format files to calculate differential expression for me? It’s not possible to manually handle the cell types, because of the number of human cell types in our research is more than 800.enter link description here
