Question: Receiving low query covers for blast searches on genome files, but not transcript files
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2.6 years ago by
Jacob10 wrote:

I'm performing blast searches with only exon regions for certain genes against various genomes(for different organisms). For example, I will blast regions 1..367, 368..444, 445..828 of the gene ACAN( against an organisms genome such as

For a lot of my blast searches, I'm only receiving hits that have a max query cover of about 30-40. If i perform the same blast searches on the transcript file

On almost all my blast searches I am receiving hits that have a query cover around 80-100

What could be the reason for this. Exons should be completely connected within the genome shouldn't they? Why would blasting against the rna transcript files return hits with higher query covers than blasting against the genome?

rna-seq transcript blast genome • 660 views
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