Hi, all I am new in bioinformatics and need your help on a simple question. I got two fastq files (forward.fastq and reverse.fastq) of one sample by RNA-seq. Then, I used fastqc to treat with two fastq files, separately and I could calculate number of raw reads, Q30, GC content of each fastq file. So how to calculate number of raw reads, Q30, GC content for this sample? Should I get the sum of raw read number of two fastq files for raw reads number of this sample, the mean value of Q30 or GC content of two fastq files for Q30 or GC content of this sample?
I was so confused about that. Thank you for your help.