Hi. I am newby at bioinformatics. I have done an experiment: three replicates of Control, three replicates of Treatment 1 and three replicates of Treatment 2. I had a service provider do RNA-seq with some bioinformatic analysis. What I received was an overall analysis even if I had told the service provider that this was actually two experiments with a common control. Now, how do I separate the analyses between Control and Treatment 1 AND Control and Treatment 2? Will I do again de novo assembly for each? I suspect that separating them in terms of DEGs is a lot easier. What about annotation?
Question: Splitting the overall RNA-seq data
2.4 years ago by
aeserrano • 40
aeserrano • 40 wrote:
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