Question: Using cnvkit for targeted amplicon sequencing
gravatar for ingrid.schulman
8 weeks ago by
ingrid.schulman0 wrote:

Hi, I'm trying to use cnvkit python package to calculate copy numbers for targeted amplicon sequenced data. I have cancer samples and normal samples and would like to do a paired reference, is this possible to do with cnvkit and how do I do it? In the documentation I only find examples for TAS without the usage of normal samples.

example: reference *.targetcoverage.cnn --no-edge -o ref-tas.cnn

ADD COMMENTlink modified 7 weeks ago by Eric T.1.8k • written 8 weeks ago by ingrid.schulman0
gravatar for Eric T.
7 weeks ago by
Eric T.1.8k
San Francisco, CA
Eric T.1.8k wrote:

Yes, the pipeline is nearly the same, but skipping the antitarget parts in coverage, fix, and reference.

With the batch command, use the option -m amplicon to run the whole workflow with reasonable defaults.

In the examples here, you can include a pool of normal samples with the -n flag, as in the standard pipeline.

ADD COMMENTlink written 7 weeks ago by Eric T.1.8k
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