Question: ValueError: invalid literal for int() with base 10: 'start' computeMatrix of deeptools
0
gravatar for salamandra
15 months ago by
salamandra170
salamandra170 wrote:

When running the command with deeptools:

DIR=~/TESTING/day2_fos20_intersection
computeMatrix scale-regions -S path/HDF_day20.bw \
path/HUVEC_day2.bw \
-R $DIR/day2_fos20.bed $DIR/day2.bed $DIR/fos20.bed -b 4000 -a 4000 --regionBodyLength 5000 --missingDataAsZero -bs 50 -out HDFd20vsHUVd2_matrixscale.gz

I get the error:

ValueError: invalid literal for int() with base 10: 'start'

What am I doing wrong?

chip-seq deeptools • 1.2k views
ADD COMMENTlink modified 10 days ago by RamRS17k • written 15 months ago by salamandra170

No header. I have some Chr17_gl00205_random though...don't know if that is a problem

ADD REPLYlink written 15 months ago by salamandra170

Please post the first 10 or so lines of your BED files.

ADD REPLYlink written 15 months ago by Devon Ryan84k
1

I have to ask you sorry. you were so right!

ADD REPLYlink written 15 months ago by salamandra170

No worries!

ADD REPLYlink written 15 months ago by Devon Ryan84k
2
gravatar for venu
15 months ago by
venu5.6k
Germany
venu5.6k wrote:

Does your bed files have a header? If yes, try removing and run it again.

ADD COMMENTlink written 15 months ago by venu5.6k

They don't have headers, I checked ;)

ADD REPLYlink written 15 months ago by salamandra170

Are you sure? By header I meant chr start end. I added this header to my bed file and used the command you mentioned. It is exactly giving the same error

ValueError: invalid literal for int() with base 10: 'start'

Without header it works completely fine.

ADD REPLYlink modified 15 months ago • written 15 months ago by venu5.6k
0
gravatar for udi.landau
11 days ago by
udi.landau10
udi.landau10 wrote:

In my case I had NA`s in the bed file

ADD COMMENTlink written 11 days ago by udi.landau10
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