Question: Why product sizes differ so much among different single cell RNA sequencing datasets?
gravatar for Snow Yeung
23 months ago by
Snow Yeung20
Guangzhou, China
Snow Yeung20 wrote:

Hi there,

Recently I want to download some scRNA-seq data from public database. During searching, I found that some scRNA-seq datasets may have around 2~3Gb for one sample (no matter PE or SE), but some just around 30Mb~300Mb. It's werid for me since I just familiared with bulk cells RNA sequencing and these runs usually have serveral Gb sizes per sample. Are the size around several Mb normally for one scRNA-seq sample data? And how to analyze these small size scRNA-seq datasets? Any help would be apperciate!

rna-seq • 598 views
ADD COMMENTlink modified 23 months ago • written 23 months ago by Snow Yeung20

Maybe the (simple and obvious) reason is just multiplexing more samples?

There are two other possible factors: there are huge variations in how much RNA particular cells have; and single cell RNA yields low amount of RNA, sequencing at great depth is only likely to increase duplication level.

ADD REPLYlink modified 23 months ago • written 23 months ago by h.mon25k
Please log in to add an answer.


Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 656 users visited in the last hour