I'm having essentially a similar error output to the original asker in this thread (https://www.biostars.org/p/229783/#260571), but mine has something to with GnuTLS, so my output starts with:
Warning: [blastn] Unavailable feature GNUTLS
Critical: [blastn] Unavailable feature GNUTLS
Error: (303.7) [blastn] [URL_Connect; https://www.ncbi.nlm.nih.gov:443/Service/dispd.cgi?service=blast4&address=10.1.153.186&platform=x86_64-apple-darwin15.6.0] Failed to connect: Not supported
My input line is:
blastn -db nt -query $FILE -out theten -entrez_query "$EQ" -outfmt 17 -max_target_seqs 10 -remote
I've used brew to double check the the most recent versions of blast+, GnuTLS (and its dependencies; GMP and nettle) are up to date.
Does anyone have any ideas where I'm going wrong?
Thanks and kind regards