Entering edit mode
6.8 years ago
Megan
▴
50
Hi, I am trying to do transcript reconstruction and quantification using StringTie on BAMs generated by STAR. According to https://ccb.jhu.edu/software/stringtie/#install, modifying the tag HI in the BAM alignment file to start at 0 (as previously required with STAR produced alignment files) is no longer needed starting at v1.1.0 release 10/19/2015. I came across this after having generated BAMs using STAR with --outSAMattrIHstart 0 option. And StringTie finished successfully using the BAMs generated by STAR. I'm really concerned if there are any underlying mistakes doing so. Thanks!
