I have done metagenomic sequencing on Illumina : amplicon of 16s rRNA (V3-V4) and want to analyse the functional genes by utilising prokaryotic dynamic programming gene defining algorithm (PRODIGAL) /KEGG and KAAS but not very familiar to the system of clustering the fastQ files and running through these softwares. I am seeking for simplified basic step by step advise
How to cluster 16S fastQ files and run on Prodigal to determine the genes in metagenomic sample?. Available from: https://www.researchgate.net/post/How_to_cluster_16S_fastQ_files_and_run_on_Prodigal_to_determine_the_genes_in_metagenomic_sample [accessed Jul 14, 2017].