Question: (Closed) CRISPR vector redesign
0
gravatar for Saeid Kadkhodaei
3.3 years ago by
IUT
Saeid Kadkhodaei90 wrote:

Hi there,

What is the best option for gRNA replacement in a CRISPR vector, considering there is no suitable RE even a few thousands bp around and the vector is 18kb in size. The vector was already constructed through USER cloning, but I'm thinking about other possible alternatives like MOE-PCR.

Thank you.

vector grna crispr • 554 views
ADD COMMENTlink written 3.3 years ago by Saeid Kadkhodaei90

This is not a bioinformatics question. You should try on biology stack exchange or similar forum for wet lab questions.

ADD REPLYlink written 3.3 years ago by Jean-Karim Heriche23k

Hello Saeid Kadkhodaei!

We believe that this post does not fit the main topic of this site.

Not a bioinformatics question

For this reason we have closed your question. This allows us to keep the site focused on the topics that the community can help with.

If you disagree please tell us why in a reply below, we'll be happy to talk about it.

Cheers!

ADD REPLYlink written 3.3 years ago by Jean-Karim Heriche23k
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