hi
i having R1, R2 read sequence fastq file, then joined pair end reads using QIIME, the out put file join.fastq. then i spliting the file, i got the output file join.fna file. in this file header line showing as bellow
>SE_0 M04635:15:000000000-AWWD2:1:1101:18457:1772 1:N:0:16 orig_bc=AAAAAAAAAAAA new_bc=AAAAAAAAAAAA bc_diffs=0
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATACCGCGTGGGTGT
ok.....
i need to remove from :
1:N:0:16 orig_bc=AAAAAAAAAAAA new_bc=AAAAAAAAAAAA bc_diffs=0
......this part from above line........
plz help
Don't remove that part since you will lose information about which read file (R1/R2) the data came from.